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2 edition of investigation of methods used to determine the growth of thiobacillus ferrooxidans found in the catalog.

investigation of methods used to determine the growth of thiobacillus ferrooxidans

Brian Michael Plaunt

investigation of methods used to determine the growth of thiobacillus ferrooxidans

by Brian Michael Plaunt

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Published by Laurentian University, Department of Biology in Sudbury, Ont .
Written in English


Edition Notes

Statementby B. M. Plaunt.
The Physical Object
Paginationviii, 41 p. :
Number of Pages41
ID Numbers
Open LibraryOL20006747M

The carbon sources that were used for the growth of Acidithiobacillus thiooxidans are Glucose, Sucrose, Fructose, Raffinose, D-sorbitol, Galactose, Lactose, Maltose, Rham-manose and Mannose. The optical density of the above carbon sources was taken at nm, af 48, 72, and 96 and hours, to check the growth of isolates. Studies on the growth of Thiobacillus ferrooxidans Studies on the growth of Thiobacillus ferrooxidans Tuovinen, Olli; Kelly, Donovan 98 98 1 1 Olli H. Tuovinen Donovan P. Kelly Department of Microbiology Queen Elizabeth College London Department of Agricultural Biochemistry Waite Agricultural Research Institute Glen Osmond South Australia Australia Abstract 1.

Growth kinetics of Thiobacillus ferrooxidans isolated from arsenic mine drainage. Biooxidacion de concentrados de arsenopirita por acidithiobacillus ferrooxidans en erlenmeyer agitados Leaching of heavy metals from contaminated soil by using Thiobacillus ferrooxidans or T. Bioleaching of metal sulfides is an interfacial process where adhesion and subsequent biofilm formation are considered to be crucial for this process. In this study, adhesion and biofilm formation by several acidophiles (Acidithiobacillus, Leptospirillum and Sulfobacillus) isolated from different biotopes with sulfide ores in Armenia were studied. Results showed that: (1) these bacteria adhere Cited by: 1.

In the present work, the streaming potential of A. ferrooxidans and pyrite was measured in two environments: fresh and saline water (water with 35 g/L of NaCl) at different pH values. Also, attachment kinetics of A. ferrooxidans to pyrite was studied in fresh and saline water at pH 4. The results show that A. ferrooxidans and pyrite had lower streaming potentials (comparing absolute values) in Cited by: 1. Abstract. The present article illustrates the increased interest which is manifested in the microorganisms, Thiobacillus ferrooxidans, involved in the biohydrometallurgical extraction wide varieties of problems currently studied are very important in order to gain a better understanding about the factors which are governing the growth of microorganisms, and as a consequence, the Cited by:


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Investigation of methods used to determine the growth of thiobacillus ferrooxidans by Brian Michael Plaunt Download PDF EPUB FB2

Thiobacillus ferrooxidans is a gram-negative, highly acidophilic (pH to ), autotrophic bacterium that obtains its energy through the oxidation of ferrous iron or reduced inorganic sulfur compounds.

It is usually dominant in the mixed bacterial Cited by: For instance, the corrosion current in the presence of Thiobacillus ferrooxidans bacteria after 72 hours and at pH of 1 is significantl y higher than that in DIW ( nA vs.

nA). The initial. Further investigation using non-invasive methods for in situ analysis of sulfur globules (such as sulfur K-edge XANES or Laser Raman spectroscopy) will be needed in order to unveil the exact chemical nature of the sulfur species generated by A.

ferrooxidans in its extracellular sulfur globules and whether they are different from the composition Cited by: Thiobacillus is a genus of Gram-negative Betaproteobacteria.

Thiobacilus thioparus is the type species of the genus, and the type strain thereof is the Starkey T strain, isolated by Robert Starkey in the s from a field at Rutgers University in the United States of over 30 "species" have been named in this genus since it was defined by Martinus Beijerinck in(the first Class: Betaproteobacteria.

Acidithiobacillus is a genus of the Acidithiobacillia in the "Proteobacteria".Like all "Proteobacteria", Acidithiobacillus spp. are members of this genus were classified as Thiobacillus spp., before they were reclassified in Acidithiobacillus ferrooxidans (basonym Thiobacillus ferrooxidans) can be isolated from iron-sulfur minerals such as pyrite deposits, oxidising Class: Acidithiobacillia.

The attachment of Thiobacillus ferrooxidans was found to be dependant on three factors, particle size (Shrihari et al.,Murthy and NatarajanShrihari et al. ), exposure time to the mineral surface of the bacteria and the effect of agitation during incubation (Murthy and Natarajan, ).Cited by:   A bioprocessing approach for the extraction of base, nuclear and precious metals from refractory and lean grade ores has been reviewed in this paper.

Characteristic morphological features ofThiobacillus ferrooxidans, the organism which has been extensively used for biooxidation of sulphide ores have been discussed. Mechanisms of chemoautotrophy and mineral oxidation have been : Sanghamitra Mukhopadhyay, K A Natarajan.

Mixotrophic Growth of a Thiobacillus ferrooxidans Strain Article (PDF Available) in Applied and Environmental Microbiology 47(3) April with 62 Reads How we measure 'reads'.

Kurosawa H, Maeda K, Yamamito E, Nakamura K, Amano Y. Growth of Thiobacillus ferrooxidans utilized thiosulfate through the oxidation route with iron recycling.

J General Appl Microb – Google Scholar. Lacey DT, Lawson F. Kinetics of liquid phase oxidation of acid FeSO 4 by the bacterium Thiobacillus by: 3. Other articles where Thiobacillus is discussed: bacteria: Autotrophic metabolism: Thiobacillus oxidizes thiosulfate and elemental sulfur to sulfate, and A.

ferrooxidans oxidizes ferrous ions to the ferric form. This diverse oxidizing ability allows A. ferrooxidans to tolerate high concentrations of many different ions, including iron, copper, cobalt, nickel, and zinc.

The proposed method is more simple and rapid than the direct cell counting and protein assay that are usually used to estimate the cell growth of Thiobacillus ferrooxidans. Using the modified turbidity method, the growth rates of Thiobacillus ferrooxidans in 9K medium containing 0 to 1, g/m 3 of Tw B or polyethyleneglycol were.

metabolic energy. Thiobacillus, by its production of sulphuric acid is involved in the destruction of concrete sewers and the acid corrosion of metals (2).

Thiobacillus Agar is a modification of formulation described by Starkey (1) and is used for the isolation and maintenance of Thiobacillus Size: 38KB.

thiobacillus (ˌθaɪəʊbəˈsɪləs) n, pl -li (-laɪ) (Biology) a rod-shaped bacterium in the genus Thiobacillus that oxidizes sulphur and is found living in sewage, water, and soil ThesaurusAntonymsRelated WordsSynonymsLegend: Switch to new thesaurus Noun 1. thiobacillus - small rod-shaped bacteria living in sewage or soil and oxidizing sulfur.

In this connection the culture of T. ferrooxidans was used in order to determine the posibility of using both standard and modified Thiobacillus agar for the isolation of other species of Thiobacilli genera.

Unfortunately no growth of T. ferrooxidans was observed on both tested agars. ConclusionsCited by: Natural population of bacteria on Bor mine site.

Mesophiles, acidithiobacillus. Pronk JT, et al. Energy transduction by anaerobic ferric iron respiration in Thiobacillus ferrooxidans. Appl. Environ. Microbiol. Irazabal N, et al. Genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC J. Bacteriol.PubMed: D.

ferrooxidans oxidizes FeSO4 to Fe3+ + H2SO4 Answer Feedback: T. ferrooxidans oxidizes FeSO4 to Fe3+ + H2SO4 in the oxidation pond. ferrooxidans is an aerobe, so the pond must be oxygenated. The process of leaching copper from ore is cyclical because T.

ferrooxidans oxidizes 4 to Fe3+ + H2SO4. Other articles where Acidithiobacillus ferrooxidans is discussed: bacteria: pH: sulfide deposits are attacked by A. ferrooxidans to generate sulfuric acid, which lowers the pH to or even However, acid tolerance of A.

ferrooxidans applies only to sulfuric acid, since these bacteria die when exposed to equivalent concentrations of other acids such as hydrochloric acid. An acidophilic strain was isolated from drainage in Shanxi coal mine, after 16SrDNA amplification and sequencing analysis, it was identified to be Acidithiobacillus ferrooxidans.

The ferrous ion oxidation rate of At.f and pH value were studied during its growth, the results showed that, the strain entering into the exponential growth phase needed about 30 hours, and the oxidation rate was more Author: Ju Fang Xiao, Song Yan Qin, Peng Huang, Li Na Guo, Yi Chun Chen.

Laboratory experiments were conducted to determine the effects of As(III) and As(V) on Thiobacillus ferrooxidans. Even though Thiobacillus ferrooxidans is a widely studied bacterium due to its use in bioleaching of mining ores, new characteristics have been discovered through this study.

ferrooxidans is proposed. Thiobacillusferrooxidans inhabits drainage in acid mines and is used for bacterial leaching. Attention to bacterial leachinghasincreasedin recentyearsbecauseofits applica-tion to low-gradeores. However,there is oneweakpoint in T.

ferrooxidans when used for bacterial leaching under severe acidic conditions in which.The oxidation of ferrous iron by Acidithiobacillus ferrooxidans in the pH range – was characterized.

In order to measure the rate of bacterial oxidation of ferrous iron with A. ferrooxidans in this high pH range, a novel experimental methodology was developed.Isolation and Characterization of Thiobacillus 75 polymorphism of these bacteria have been noted and explained by several workers (Kuenen et al., ; Muyzer and Uitterlinden, ; Leduc and Ferroni, ; Chisholm et al., ; Rawlings, ; Frattini et al., ; Ageeva et al., ; Ito et al., ).

Keeping this concept into view, the objective of the investigation was to isolate a.